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EcoToxChip (qPCR) results file(s) upload

Please upload one or several qPCR files with Ct values produced using EcoToxChip below. For first time users, you are advised to first explore the tools using one of our example datasets.

  • The same well ID must be used across all files;
  • All files must have the same number of columns (two columns);
  • Values should represent raw Ct scores of qPCR;
  • Missing values need to be written as "NA" and not left empty or blank;
  • All files must have the same number of rows (e.g., 384 rows);
  • All files should be uploaded as text (.txt) or CSV (.csv) format;
  • At most 50 qPCR files can be uploaded.
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Click here to view a sample input file of the 384-well EcoToxChip qPCR plate.
Example qPCR Data
AnalysisOrganismDescription
EcoToxChip-Avian-v1
Differential expression (real data) C. japonica (Japanese quail) Chlorpyrifos exposure study with 15 samples (5 controls, 5 medium and 5 high dose samples). (Download Original study)
EcoToxChip-Avian-v1
Differential expression (real data) C. japonica (Japanese quail) Ethinylestradiol exposure study with 15 samples (5 controls, 5 medium and 5 high dose samples). (Download Original study)
EcoToxChip-Minnow-v1
Differential expression (real data) P. promelas (Fathead minnow) Benzo(a)pyrene exposure study with 6 samples (3 solvent controls, and 3 high dose samples). (Download Original study)
EcoToxChip-Avian-v1
Dose-response (real data) C. japonica (Japanese quail) PCB exposure study with five doses and a control (0, 0.1, 1, 10, 100, 300 ppb). (Download Original study)
EcoToxChip-Avian-v0.1
Differential expression (synthetic data) C. japonica (Japanese quail) Ethinylestradiol exposure study. This is a synthetic dataset to perform default testing and analysis. (Download Original study)
EcoToxChip-Minnow-v1 (alarming)
Differential expression (synthetic data) P. promelas (Fathead minnow) Benzo(a)pyrene exposure study with 6 samples (3 solvent controls, and 3 high dose samples). This is a manipulated version of the original dataset to examine analysis when most of the genes are responsive. (Download Original study)
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